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immunostaining cd68  (Bio-Rad)


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    Bio-Rad immunostaining cd68
    Fig. 1. KUS121 attenuated atherosclerosis progression in vivo. (A) Scheme of experimental protocol to assess the effect of KUS121 on atherosclerosis progression. (B) Representative images of HE staining in aortic sinuses and (C) quantitative analysis of plaque areas (n = 15 per group). Scale bar: 200 μm. (D) Representative images of en face ORO staining of whole thoracic aorta and (E) quantification of atherosclerotic lesions in aortic arches and descending aortas (n = 17 per group). Scale bar: 2 mm. (F) Representative images of <t>CD68</t> staining in aortic sinuses and (G) quantification of <t>CD68-positive</t> areas in atherosclerotic plaques (n = 15 per group). Scale bar: 200 μm. (H) Quantification of the proportions of Chop-positive cells in different cell types and (I) representative images of Chop staining in the atherosclerotic plaques. Scale bar: 50 μm. All data is presented as mean ± SEM **p < 0.01, ***p < 0.001 by student’s t-test.
    Immunostaining Cd68, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 3115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunostaining cd68/product/Bio-Rad
    Average 96 stars, based on 3115 article reviews
    immunostaining cd68 - by Bioz Stars, 2026-03
    96/100 stars

    Images

    1) Product Images from "KUS121, a novel VCP modulator, attenuates atherosclerosis development by reducing ER stress and inhibiting glycolysis through the maintenance of ATP levels in endothelial cells."

    Article Title: KUS121, a novel VCP modulator, attenuates atherosclerosis development by reducing ER stress and inhibiting glycolysis through the maintenance of ATP levels in endothelial cells.

    Journal: Atherosclerosis

    doi: 10.1016/j.atherosclerosis.2025.119223

    Fig. 1. KUS121 attenuated atherosclerosis progression in vivo. (A) Scheme of experimental protocol to assess the effect of KUS121 on atherosclerosis progression. (B) Representative images of HE staining in aortic sinuses and (C) quantitative analysis of plaque areas (n = 15 per group). Scale bar: 200 μm. (D) Representative images of en face ORO staining of whole thoracic aorta and (E) quantification of atherosclerotic lesions in aortic arches and descending aortas (n = 17 per group). Scale bar: 2 mm. (F) Representative images of CD68 staining in aortic sinuses and (G) quantification of CD68-positive areas in atherosclerotic plaques (n = 15 per group). Scale bar: 200 μm. (H) Quantification of the proportions of Chop-positive cells in different cell types and (I) representative images of Chop staining in the atherosclerotic plaques. Scale bar: 50 μm. All data is presented as mean ± SEM **p < 0.01, ***p < 0.001 by student’s t-test.
    Figure Legend Snippet: Fig. 1. KUS121 attenuated atherosclerosis progression in vivo. (A) Scheme of experimental protocol to assess the effect of KUS121 on atherosclerosis progression. (B) Representative images of HE staining in aortic sinuses and (C) quantitative analysis of plaque areas (n = 15 per group). Scale bar: 200 μm. (D) Representative images of en face ORO staining of whole thoracic aorta and (E) quantification of atherosclerotic lesions in aortic arches and descending aortas (n = 17 per group). Scale bar: 2 mm. (F) Representative images of CD68 staining in aortic sinuses and (G) quantification of CD68-positive areas in atherosclerotic plaques (n = 15 per group). Scale bar: 200 μm. (H) Quantification of the proportions of Chop-positive cells in different cell types and (I) representative images of Chop staining in the atherosclerotic plaques. Scale bar: 50 μm. All data is presented as mean ± SEM **p < 0.01, ***p < 0.001 by student’s t-test.

    Techniques Used: In Vivo, Staining



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    Fig. 1. KUS121 attenuated atherosclerosis progression in vivo. (A) Scheme of experimental protocol to assess the effect of KUS121 on atherosclerosis progression. (B) Representative images of HE staining in aortic sinuses and (C) quantitative analysis of plaque areas (n = 15 per group). Scale bar: 200 μm. (D) Representative images of en face ORO staining of whole thoracic aorta and (E) quantification of atherosclerotic lesions in aortic arches and descending aortas (n = 17 per group). Scale bar: 2 mm. (F) Representative images of <t>CD68</t> staining in aortic sinuses and (G) quantification of <t>CD68-positive</t> areas in atherosclerotic plaques (n = 15 per group). Scale bar: 200 μm. (H) Quantification of the proportions of Chop-positive cells in different cell types and (I) representative images of Chop staining in the atherosclerotic plaques. Scale bar: 50 μm. All data is presented as mean ± SEM **p < 0.01, ***p < 0.001 by student’s t-test.
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    Fig. 1. KUS121 attenuated atherosclerosis progression in vivo. (A) Scheme of experimental protocol to assess the effect of KUS121 on atherosclerosis progression. (B) Representative images of HE staining in aortic sinuses and (C) quantitative analysis of plaque areas (n = 15 per group). Scale bar: 200 μm. (D) Representative images of en face ORO staining of whole thoracic aorta and (E) quantification of atherosclerotic lesions in aortic arches and descending aortas (n = 17 per group). Scale bar: 2 mm. (F) Representative images of <t>CD68</t> staining in aortic sinuses and (G) quantification of <t>CD68-positive</t> areas in atherosclerotic plaques (n = 15 per group). Scale bar: 200 μm. (H) Quantification of the proportions of Chop-positive cells in different cell types and (I) representative images of Chop staining in the atherosclerotic plaques. Scale bar: 50 μm. All data is presented as mean ± SEM **p < 0.01, ***p < 0.001 by student’s t-test.
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    Perivascular meningeal lymphocytes and scattered parenchymal lymphocytes demonstrate positive staining with antibody to CD3 (T-cell lymphoid marker). The vast majority of lymphocytes seen were T cells (CD3 <t>immunostain,</t> original magnification ×100).
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    Image Search Results


    Fig. 1. KUS121 attenuated atherosclerosis progression in vivo. (A) Scheme of experimental protocol to assess the effect of KUS121 on atherosclerosis progression. (B) Representative images of HE staining in aortic sinuses and (C) quantitative analysis of plaque areas (n = 15 per group). Scale bar: 200 μm. (D) Representative images of en face ORO staining of whole thoracic aorta and (E) quantification of atherosclerotic lesions in aortic arches and descending aortas (n = 17 per group). Scale bar: 2 mm. (F) Representative images of CD68 staining in aortic sinuses and (G) quantification of CD68-positive areas in atherosclerotic plaques (n = 15 per group). Scale bar: 200 μm. (H) Quantification of the proportions of Chop-positive cells in different cell types and (I) representative images of Chop staining in the atherosclerotic plaques. Scale bar: 50 μm. All data is presented as mean ± SEM **p < 0.01, ***p < 0.001 by student’s t-test.

    Journal: Atherosclerosis

    Article Title: KUS121, a novel VCP modulator, attenuates atherosclerosis development by reducing ER stress and inhibiting glycolysis through the maintenance of ATP levels in endothelial cells.

    doi: 10.1016/j.atherosclerosis.2025.119223

    Figure Lengend Snippet: Fig. 1. KUS121 attenuated atherosclerosis progression in vivo. (A) Scheme of experimental protocol to assess the effect of KUS121 on atherosclerosis progression. (B) Representative images of HE staining in aortic sinuses and (C) quantitative analysis of plaque areas (n = 15 per group). Scale bar: 200 μm. (D) Representative images of en face ORO staining of whole thoracic aorta and (E) quantification of atherosclerotic lesions in aortic arches and descending aortas (n = 17 per group). Scale bar: 2 mm. (F) Representative images of CD68 staining in aortic sinuses and (G) quantification of CD68-positive areas in atherosclerotic plaques (n = 15 per group). Scale bar: 200 μm. (H) Quantification of the proportions of Chop-positive cells in different cell types and (I) representative images of Chop staining in the atherosclerotic plaques. Scale bar: 50 μm. All data is presented as mean ± SEM **p < 0.01, ***p < 0.001 by student’s t-test.

    Article Snippet: Following antibodies were purchased and used for immunostaining: CD68 (FA-11, Bio-Rad, MCA1957, 1:200), Actin, α-Smooth Muscle (1A4, Sigma-Aldrich, F3777, 1:500), CD31 (2H8, Merck Millipore, MAB1398Z, 1:200), CD31 (MEC13.3, BD Pharmingen, 550274, 1:400), Icam1 (YN1/1.7.4, BioLegend, 116101, 1:200), Chop (F-168, Santa Cruz, sc-575, 1:100), NF-κB (C-20, Santa Cruz, sc-372, 1:200) and Cleaved Caspase-3 (Asp175, Cell signaling technology, #9661, 1:200).

    Techniques: In Vivo, Staining

    Perivascular meningeal lymphocytes and scattered parenchymal lymphocytes demonstrate positive staining with antibody to CD3 (T-cell lymphoid marker). The vast majority of lymphocytes seen were T cells (CD3 immunostain, original magnification ×100).

    Journal: Case Reports in Pathology

    Article Title: Dual Pathology in Rasmussen's Encephalitis: A Report of Coexistent Focal Cortical Dysplasia and Review of the Literature

    doi: 10.1155/2012/569170

    Figure Lengend Snippet: Perivascular meningeal lymphocytes and scattered parenchymal lymphocytes demonstrate positive staining with antibody to CD3 (T-cell lymphoid marker). The vast majority of lymphocytes seen were T cells (CD3 immunostain, original magnification ×100).

    Article Snippet: Distributed primarily within the cortex were small collections of microglial nodules, highlighted with an CD68 immunostain (1 : 60 dilution, Dako, Carpenteria, CA, USA) ( ).

    Techniques: Staining, Marker